For biological and medical applications fluorescence microscopes are used to observe certain parts of living cells and tissues with help of fluorophores. One or more fluophores are added to the parts of the specimen to be observed. When the fluorophores are exposed to so-called exicitation light, the excitation energy is absorbed by the fluorophore that start emitting so-called emission light that can be observed by the microscope. One can distinguish tumor cells from other cells, follow biological processes or prove the presence or absence of antibodies, etc
The B+ series microscope for fluorescence comes in two versions. Two models are equipped with 4 monochromatic LEDs and two other models are equipped with a 100 W mercury vapor illuminator
The microscopes are supplied with fluorescence filters and dichroic mirrors for excitation in the blue spectral region, green, violet and ultraviolet
| MODELS | Plan PL 4x/10x/S40x/S100x IOS objectives | Plan fluarex PLF 4x/10x/S40x/S100x IOS objectives | Epi-fluorescence 4x NeoLED excitation | Epi-fluorescence 100 W Hg excitation | |
| 86.179‑LED | • | • |  | ||
| 86.180‑LED | • | • | |||
| 86.178‑LED | • | • | |||
| 86.181‑LED | • | • |
The Fluarex objectives are suitable for fluorescence excitation in the violet and ultraviolet region 4 NeoLEDTM fluorescence: Blue (EX 460 to 470 nm, EM > 515 nm), Green (EX 520 to 530 nm, EM > 590 nm),
Violet (EX 395 to 415 nm, EM 425 nm) and Ultraviolet (EX 355 to 375 nm, EM > 455 nm)
EYEPIECES
Wide field WF10x/20 mm eyepieces
HEADS
Trinocular 360° rotatable head with 30° inclined tubes. One tube is equipped with diopter adjustment and the interpupillary distance is adjustable between 48 to 76 mm. Trinocular light split 100:0/0:100
NOSEPIECE
Nosepiece for maximum 5 objectives and mounted on ball bearings
OBJECTIVES
• The 86.179-LED and86.178-LED models are supplied with plan 4x/0.10, 10x/0.25, S40x/0.65 and S100x/1.25 oil immersion infinity corrected IOS DIN objectives
• The 86.180-LED and 86.181-LED models are supplied with plan fluarex 4x/0.10, 10x/0.25, S40x/0.65 and S100x/0.90 infinity corrected IOS DIN objectives.
The plan fluarex objectives allow observation in violet and ultraviolet excitation
All S40x and S100x oil immersion objectives are spring loaded
All optics have an anti-reflection coating for maximum light throughput and are anti-fungus treated
FOCUSING
Coaxial coarse and fine adjustments, 200 graduations, 1 μm per graduation, 200 μm per rotation, total travel approximately 23 mm. Supplied with an adjustable rack stop to prevent damage to sample and objectives. The coarse adjustments are equipped with friction control
STAGE
125 x 135 mm stage with 75 x 35 mm X-Y mechanical stage. With Vernier 0.1 mm scale. Removable slide holder. Stages with heating elements and temperature PID controller are also available
CONDENSER
Height adjustable Abbe condenser N.A. 1.25 with iris diaphragm and filter holder
TRANSMITTED ILLUMINATION
Adjustable 3 W LED illumination with 3 rechargeable batteries. Batteries for cordless operation. All models have an integration 100 - 240 V power supply
FLUORESCENCE ATTACHMENT
Epi-illumination with 4 LEDs for fluorescence excitation from 460 to 470 nm (blue), 520 to 530 nm (green), 390 to 400 (violet) and 360 to 370 nm (ultraviolet). External 100-240 V power supply. Epi-illumination 100 W mercury vapor lamp. External 110 or 230 V power supply. The fluorescence attachment are equipped with dichroic mirrors and fluorescence filters for blue, green, violet and ultra-violet excitation
PACKAGING
Supplied with power cord, dust cover, a spare fuse, user manual and 5 ml immersion oil for models with S100x objective. Supplied with orange UV protection shield. All packed in a polystyrene box
86.575
86.573
80.882
86.574
80.839
86.891
86.893
86.894
86.897
AE.5699
86.900
86.511
86.513
86.514
86.517
86.519
86.520
86.521
86.413
86.414
86.417
86.420
86.313
86.314
86.317
86.320
86.550
86.559
86.613
86.615
86.617
86.820
86.860
86.889
86.101
AE.1110
AE.1111
AE.5130
AE.5040
AE.5025
AE.5202
AE.5203
AE.5204
AE.5205
AE.5207
AE.5227
AE.5365
PB.5155
PB.5165
PB.5168
PB.5245
PB.5255
PB.5274
PB.5275
86.172
AE.5168-B
86.552
86.554
86.556
86.558
