Highlights

  • Life Science applications
  • Observation of tissue cultures, living cells
  • Brightfield, phase contrast and fluorescence
  • Long working distance IOS plan achromatic objectives
  • Revolving quintuple nosepiece
  • 5 W LED transmitted illumination
  • 100 W mercury vapor lamp for fluorescence
Models
Specifications
Accessories
Downloads

Oxion Inverso

MODELS LWD Plan 10x/20x/40x objectives LWD Plan phase 10x/20x/40x objectives LWD Plan Fluarex 10x/20x/40x objectives LWD Plan Fluarex 10x and plan phase fluarex 20x/40x objectives 2-position filter block 4-position filter block Mechanical stage  
OX.2003‑PL
OX.2053‑PL
OX.2003‑PLPH
OX.2053‑PLPH
OX.2253‑PLF  
OX.2453‑PLF  
OX.2253‑PLPHF
OX.2453‑PLPHF

Fluarex objectives are made of low-absorption glass for fluorescence applications
Fluarex is a trademark of Euromex microscopen bv
LWD = long working distance 

EYEPIECES
Pair of DIN HWF plan 10x/22 mm eyepieces

HEADS
• Trinocular 45° inclined tubes with extended low and high positions for eyepieces
• One diopter adjustment on left eyepiece
• Interpupillary distance of 54 to 75 mm
• Trinocular head with 100-0 % and 80-20 % positions

NOSEPIECES
Revolving quintuple nosepiece on ball-bearings

OBJECTIVES FOR BRIGHTFIELD 
(models OX.2003-PL and OX.2053-PL)

• Infinity color corrected long working distance plan 10x/0.25, 20x/0.40, 40x/0.60 IOS objectives
• An optional 60x/0.70 IOS objective is also available

OBJECTIVES FOR PHASE CONTRASTOX.9190r
(models OX.2003-PLPH and OX.2053-PLPH)

Infinity color corrected long working distance plan phase 10x/0.25, 20x/0.40, 40x/0.60 IOS objectives 

(Photo: OX.9190 Slider with 4x/10x and 20x/40x phase contrast annuli)

OBJECTIVES FOR BRIGHTFIELD AND FLUORESCENCE MICROSCOPY
(models OX.2253-PLF and OX.2453-PLF)

• Infinity color corrected long working distance plan Fluarex 10x/0.30, 20x/0.45, 40x/0.65 IOS objectives
• An optional plan Fluarex 60x/0.75 IOS objective is also available

OBJECTIVES FOR PHASE CONTRAST AND FLUORESCENCE MICROSCOPY
(models OX.2253-PLPHF and OX.2453-PLPHF)

Infinity color corrected long working distance plan fluarex 10x/0.30 and plan phase fluarex 20x/0.45 and S40x/0.65 IOS objectives. 

All objectives corrected for 1.2 mm thickness. 
All optics are anti-fungus treated and anti-reflection coated for maximum light throughput 

OX.9599rSTAGE
Stage 250 x 230 mm equipped with a coaxial mechanical 120 x 78 mm X-Y stage, delivered with:

• metallic insert for Ø 35 mm cell culture dish dishes
• 76 x 26 mm slide holder
• glass insert with hole

optional universal cell culture dish holder
(Photo: OX.9599 Universal cell structure dish holder)

CONDENSER
• N.A. 0.30 condenser with iris diaphragm. Working distance 72 mm
• The models for the phase contrast OX.2003-PLPH, OX.2053- PLPH, OX.2253-PLPHF and OX.2453-PLPHF are delivered with a slider with 4x/10x and 20x/40x pre-centered phase rings

FOCUSING ADJUSTMENT
Coaxial 25 mm coarse and fine adjustments, 2 μm precision and with friction adjustment

ILLUMINATION
• Transmitted 5 W LED Intensity adjustable with internal 100-240 V power supply. Fuse holder 3.15 A / 250 V. 
• The fluorescence models are equipped with a reflected 100 W HBO mercury vapor light source with external 100-240 V power supply, fuse holder 3.15 A / 250 V, UV protection shield

FILTERS FOR PHASE CONTRAST MODELS
Interference filter 550 nm green and blue filter, 45 mm diameter 

STANDARD FLUORESCENCE FILTERS
(for fluorescence models)

The fluorescence models OX.2253-PLF and OX.2453-PLF are standard supplied with two filter sets for excitation:
- in the blue spectrum, OX.9745, EX465-495 (BP) DM505 (LP) EM515-558 (BP)
- in the green spectrum, OX.9746, EX520-600 (BP) DM600 (LP) EM605-665 (BP)

The fluorescence models OX.2253-PLF and OX.2453-PLPHF equipped with a 4 positions filter rail equipped with three standard filter sets for excitation:
- in the blue spectrum, OX.9745, EX465-495 (BP) DM505 (LP) EM515-558 (BP)
- in the green spectrum, OX.9746, EX520-600 (BP) DM600(LP) EM605-665 (BP)
- in the UV spectrum, OX.9747, EX361-381 (BP) D415 (LP) EM435-495 (BP)

STANDARD GENERAL PURPOSE FLUORESCENCE FILTERS SETS

OX.9745 Blue excitation 465-495 nm OX.9745_1

FITC EX465-495 (BP) DM505 (LP) EM515-558 (BP) 
Also suitable for: Acridine Orange + DNA, Alexa Fluor 488™, Atto 488, Azami Green, BODIPY FL/pH7.2, Calcein, Calcium Green™-1, Cy2™, DiO, DyLight 488, EGFP, Emerald GFP, FAM, FITC, Fluo-4, GFP LysoTracker Green/pH 5.2, MitoTracker Green FM/MeOH, mWasabi, Oregon Green™ 488, SYBR® Green I, SYTO 9/DNA, ZsGreen




OX.9746_1

OX.9746 Green excitation 520-600 nm

Texas Red / mCherry / Alexa Fluor 594 EX520-600 (BP) DM600 (LP) EM605-665 (BP)
Also suitable for: Alexa Fluor 568™, Alexa Fluor 594™, CAL Fluor® Red 610, Cy3.5™, DyLight 594, FusionRed, Killer Red, mCherry, MitoTracker Red/MeOH, mKate2, mRFP1, ReAsH-CCPGCC, Rhodamine Red™-X, ROX, Sulforhodamine 101, Texas Red®, Texas Red®-X, X-rhod-1/Ca2+




OX.9747_1

OX.9747 UV excitation 361-381 nm

DAPI / Hoechst / Alexa Fluor 350 EX361-381 (BP) D415 (LP) EM435-495 (BP)
Also suitable for: Alexa Fluor 350™, Biosearch Blue, Coumarin, DAPI, DyLight 350, Hoechst 33258, LysoTracker Blue/MeOH







OX.9748_1

OX.9748 Violet excitation 361-389 nm

UV / DAPI Longpass, Calcofluor White EX 361-389 (BP) DM 415 (LP) EM 435 (LP)

 






PACKAGE CONTENT
• Supplied with power cord, dust cover and user manual
• Phase contrast models are supplied with green filter and alignment telescope

ISCI (INTRACYTOPLASMIC SPERM INJECTION) SET FOR OXION INVERSO

OX.2003-PLPH-ISCI

OX.2003-PLPH-ISCI_detail

(Photo: OX.2003-PLPH-ISCI Oxion Inverso with ICSI equipment 
for Intracytoplasmic Sperm Injection

TECHNICAL SPECIFICATIONS
• Perfect for inexperienced users in need of training practice as well as for professionals
• Complete manual system
• Large movement range
• Easy micropipette position at each time ‘Manual Home Position’ 
• Adjustable micropipette holder angle between 20° and 40°
• Micropipette holder fixing knob reduces vibration and easy handling of the sperm
• Easy installation and service
• 3D Hydraulic Manipulation Joysticks for smooth movement
• Pneumatic Air Injector for ICSI and HOLDING
• No service required for injector
• Identical sensitive injectors on both sides and interchangeable 
• Top quality for affordable price
• Medical Device CE mark
• Movement Range: X/Y/Z: 20 mm each, 1 mm for one complete turn of the handle
• Optional heating stage with controller 
(Photo: 
ISCI set (detail)

ISCI SET
Set includes two 3D hydraulic joysticks, two pipette setup mechanisms, one connection bar with moving handle, two air injectors with tubing and pen type micromanipulation, pipette holder, two metal plates for injectors, one set of Allen wrenches, one ruler for mechanic home position adjustment.  Unit is packed in a solid carry case

HEATING STAGE
• Heating glass stage for Euromex microscope stage with 110 mm circular hole
• The glass plate fits the circular opening of the X-Y stage and enables a flat glass object-plate with constant temperature
• Aluminium frame for superior resistance and flatness 
• Glass thickness 0.5 mm. Plate diameter 110 mm 

CONTROLLER
• 2-Channel Temperature Controller with touchscreen interface
• One controller can control up to two devices
• External temperature sensor (included) and self calibration routines allows easy calibration of the heating devices 
• On-board data logging allows to record temperature as a function of time and to log calibration events and alarms 
• The password protected controller is equipped with routines to re-calibrate the sensors against certified thermometers 

FEATURES
•Temperature range: from ambient to 60°C
• Temperature accuracy on specimen: ± 0.3°C
• 4.3’’ touch screen interface
• External Temperature sensor
• On-board Data logging to USB drive
• Alarm buzzer and External Alarm Connector
• One controller can control up to two devices
• Self calibration routines enable high temperature accuracy 

OX.6010 OX.6010
Wide field eyepiece HWF 10x/22 mm
OX.6015 OX.6015
Wide field eyepiece HWF 15x/16 mm for Oxion Inverso inverted micrososcopes
OX.6110 OX.6110
Wide field eyepiece HWF 10x/22 mm with reticle for Oxion Inverso inverted micrososcopes
OX.6099 OX.6099
Pair of eyecups for Oxion Inverso inverted micrososcopes
OX.8304 OX.8304
Plan LWD 4x/0.13 IOS objective, corrected for 1.2 mm
OX.8310 OX.8310
Plan LWD 10x/0.30 IOS objective, corrected for 1.2 mm
OX.8320 OX.8320
Plan LWD 20x/0.45 IOS objective, corrected for 1.2 mm
OX.8340 OX.8340
Plan LWD 40x/0.65 IOS objective, corrected for 1.2 mm
OX.8360 OX.8360
Plan LWD 60x/0.75 IOS objective, corrected for 1.2 mm
OX.8404 OX.8404
Plan phase LWD 4x/0.13 infinity corrected IOS, objective, working distance 10.4 mm
OX.8410 OX.8410
Plan phase LWD 10x/0.25 infinity corrected IOS, objective, working distance 7.94 mm
OX.8420 OX.8420
Plan phase LWD 20x/0.40 infinity corrected IOS, objective, working distance 7.66 mm
OX.8440 OX.8440
Plan phase LWD 40x/0.60 infinity corrected IOS, objective, working distance 3.71 mm
No image OX.8604
Plan LWD 4x/0.13 infinity corrected IOS Fluarex objective, corrected for 1.2 mm
OX.8610 OX.8610
Plan LWD 10x/0.30 infinity corrected IOS Fluarex objective, corrected for 1.2 mm
OX.8620 OX.8620
Plan LWD 20x/0.45 infinity corrected IOS Fluarex objective, corrected for 1.2 mm
OX.8640 OX.8640
Plan LWD 40x/0.65 infinity corrected IOS Fluarex objective, corrected for 1.2 mm
OX.8660 OX.8660
Plan LWD 60x/0.75 infinity corrected IOS Fluarex objective, corrected for 1.2 mm
OX.8720 OX.8720
Plan LWD Phase contrast 20x/0.40 infinity corrected IOS Fluarex objective, corrected for 1.2 mm
OX.8740 OX.8740
Plan LWD Phase contrast 40x/0.65 infinity corrected IOS Fluarex objective, corrected for 1,2 mm
OX.9148 OX.9148
Telescope for phase contrast fluorescence alignment
OX.9149 OX.9149
Telescope for phase contrast alignment for Oxion Inverso inverted micrososcopes
OX.9190 OX.9190
Slider with 4x/10x and 20x/40x phase contrast annuli for Oxion Inverso inverted micrososcope
OX.9500 OX.9500
Mechanical X-Y stage, 120 x 78 mm for Oxion Inverso inverted micrososcopes
OX.9506 OX.9506
Transparant glass insert with hole for Oxion Inverso inverted micrososcopes
OX.9508 OX.9508
Metal insert with hole for Oxion Inverso inverted micrososcopes
OX.9512 OX.9512
Insert with 76 x 26 mm slide holder and diameter 54 mm cell culture dish holder
OX.9535 OX.9535
Petri dish insert 35 mm for Oxion Inverso inverted micrososcopes
OX.9550 OX.9550
Stage extension plate
OX.9599 OX.9599
Universal cell culture dish holder
OX.9700 OX.9700
Blue filter 45 mm diameter
No image OX.9703
IR cut-off filter
OX.9985 OX.9985
5 W NeoLED™ replacement unit
OX.9750.0401 OX.9750.0401
Premium fluorescence filter set EX440BP21 DM455LP EM480BP30 optimized for CFP. Compatible with eCFP, mCFPm, Cerulean, CyPet adn SpectrumAqua. This filter set is designed for optimal signal capture of CFP and to minimize spectral bleed-through of YFP. Needs empty block OX.9749
OX.9750.0403 OX.9750.0403
Premium fluorescence filter set EX380BP50 DM410LP EM450BP60 optimized for BFP, BFP2. Compatible with AlexaFluor 350, DAPI; Hoeschst 33342 & 33258. Wide excitation filter bandwidth may cause cellular damage in live cell applications. Needs empty block OX.9749
OX.9750.0404 OX.9750.0404
Premium fluorescence filter set EX470BP40 DM500LP EM535BP50 optimized for AlexaFluor 488. Compatible with Cy2, eCFP, FITC and GPT (sapphire). Designed for both excellent brightness and contrast with greater than OD 6 at the excitation/emission crossover. Needs empty block OX.9749
OX.9750.0407 OX.9750.0407
Premium fluorescence filter set EX635BP30 DM660LP EM710BP80 optimized for AlexaFluor 647. Compatible with Cy5, TOTO-1, TOTO-3, Kodak X-Sight 650. A wide emission filter for maximum photon capture. A narrow excitation filter. Needs empty block OX.9749
OX.9750.0412 OX.9750.0412
Premium fluorescence filter set EX500BP25 DM525LP EM545BP35 optimized for YFP and for minimizing CFP bleedthrough. Compatible with AlexFluor 532, Acridine Orange (+DNA), Rhodamine Green . Needs empty block OX.9749
OX.9750.0414 OX.9750.0414
Premium fluorescence filter set EX560BP55 DM595LP EM645BP75 optimized for Texas Red. Compatible with Texas Red-X, Mito Tracker Red, Cy 3,5, HcRed, mRasberry. Wide passbands that give high brightness and contrast to red emitting fluorophores such as Texas Red. Needs empty block OX.9749
OX.9750.39001 OX.9750.39001
Fluorescence filter set EX435 (BP20) DM455 (LP) EM480 (BP30) optimzed for AmCyan, Atto 425, Cerulean, CFP, ECFP fluorophore. Needs empty filter block (OX.9749)
OX.9750.39002 OX.9750.39002
Fluorescence filter set EX480 (BP30) DM505(LP) EM535 (BP40) optimized for Acridine Orange + DNA, Alexa Fluor 488™, Atto 488, Azami Green, BODIPY FL/pH7.2, Calcein, Calcium, Green™-1, Cy2™, DiO, DyLight 488, EGFP, Emerald GFP, FAM, FITC, Fluo-4, GFP, LysoTracker Green/pH 5.2, MitoTracker Green, FM/MeOH, mWasabi, Oregon Green™ 488, SYBR® Green I, SYTO 9/DNA, ZsGreen fluorophore. Needs empty filter block (OX.9749)
OX.9750.39003 OX.9750.39003
Fluorescence filter set EX495 (BP20) DM515 (LP) EM540 (BP30) optimized for Alexa Fluor 514™, Citrine, EYFP/pH 7, F/lAsH-CCPFCC, mCitrine, Oregon Green™ 514, Rhodamine 123, Topaz. Needs empty filter block (OX.9749)
OX.9750.39004 OX.9750.39004
Fluorescence filter set EX540 (BP25) DM565 (LP) EM605 (BP55) optimized for Alexa Fluor 546™, Alexa Fluor 555™, Atto 550, CAL, Fluor® Red 590, Cy3™, DiI, DsRed, DyLight 549, MitoTracker Orange/MeOH, Resorufin, Rhod-2, TagRFP, TAMRA tdTomato, Tetramethylrhodamine, isothiocyanate, TRITC. Needs empty filter block (OX.9749)
OX.9750.39005 OX.9750.39005
Fluorescence filter set EX540 (BP25) DM565 (LP) EM 620 (BP60) optimized TRITC-red-shifted, Ethidium homidimer-1/DNA, Propidium Iodide. Needs empty filter block (OX.9749)
OX.9750.39007 OX.9750.39007
Fluorescence filter set EX620 (BP50) DM655 (LP) EM 690 (BP50) optimized for Alexa Fluor 647™, Allophycocyanin (APC), Atto 647N, Cy5™, DiD, Draq5, DyLight 649 MitoTracker Deep Red 633/MeOH, Nile Blue, Quasar® 670, SYTO® 60, TO-PRO™-3. Needs empty filter block (OX.9749)
OX.9750.39008 OX.9750.39008
Fluorescence filter set EX420 (BP40) DM455 (LP) EM 535 (BP40) optimized for Lucifer Yellow/Tetracycline/AlexaFluor 430. Needs empty filter block (OX.9749)
OX.9750.39009 OX.9750.39009
Fluorescence filter set EX480 (BP30) DM505 (LP) EM 620 (BP60) for Acridine Orange + RNA, Di-8-ANEPPS, non-ratiometric, DiA, Ethidium Bromide, FM™ 1-43, LysoTracker Yellow HCK-123, Pulsar™ 650. Needs empty filter block (OX.9749)
AE.5120 AE.5120
Standard 23.2 mm tube for Oxion upright (revision 1) and Oxion Inverso inverted microscopes
AE.5120-2 AE.5120-2
Standard 23.2 mm tube for Oxion upright (revision 2) and Oxion Inverso inverted microscopes
AE.5130 AE.5130
Universal SLR-adapter with built-in 2x lens for standard 23.2 mm tube. Needs T2 adapter
AE.5025 AE.5025
T2 ring for Nikon D SLR-digital camera
AE.5040 AE.5040
T2 ring for Canon EOS SLR-digital camera
OX.9833 OX.9833
Photo port adapter with 0.33x lens for Oxion (revision 2) and Oxion Inverso microscopes and 1/3 inch camera with C-mount
OX.9850 OX.9850
Photo port adapter with 0.5x lens for Oxion (revision 2) and Oxion Inverso microscopes and 1/2 inch camera with C-mount
AE.1385 AE.1385
100 W HBO mercury lamp for microscopes with fluorescence attachment
AE.3199 AE.3199
Glass fuses 3.15A 250V, 10pcs
PB.5155 PB.5155
Microscope slides 76 x 26 mm, ground edges, 50 pieces per pack
PB.5165 PB.5165
Cover glasses 18 x 18 mm, 0.13-0.17 mm, 100 pieces
PB.5168 PB.5168
Cover glasses 22 x 22 mm, 0.13-0.17 mm, 100 pieces
PB.5245 PB.5245
Lens cleaning paper, 100 sheets per pack
PB.5274 PB.5274
Iso propyl alcohol 99%, 200 ml
PB.5275 PB.5275
Cleaning kit: lens fluid, lint free lens tissue paper, brush, air blower, cotton swabs
No image AE.5127
Standard 23.2 mm tube for Oxion (revision 1)
No image OX.9810
C-mount 1x photo adapter for Oxion (revision 2)
No image OX.5700-ADD
Oxion Ergo Head Add-on price
No image AE.3197-ADD
Oxion ceramic stage Add-on price
No image OX.9161
Slider with 10x/20x and 40x phase contrast annuli for Oxion Inverso inverted micrososcopes
OX.9507 OX.9507
Transparant glass insert without hole for Oxion Inverso inverted micrososcopes
OX.9510 OX.9510
Metal insert for microplates (Multiwell) for Oxion Inverso inverted micrososcopes
OX.9714 OX.9714
Blauw 550 nm interference filter 45 mm diameter for Oxion Inverso
OX.9787 OX.9787
Green 550 nm interference filter 45 mm diameter for Oxion Inverso
No image AE.8010
Heating stage with transparent glass plate for AE.8000 Micromanipulator, including digital temperature controller