Highlights

  • Life science applications
  • Observation of fluorescence in tissues and cells
  • Brightfield, phase contrast fluorescence observations
  • DIC (differential interference contrast)
  • Long working distance IOS plan semi-apochromatic Fluarex achromatic objectives
  • Revolving sextuple nosepiece
  • 5 W LED or 100 W halogen transmitted illumination
  • 100 W HBO mercury vapor fluorescence illumination
  • Backwards tilting condenser arm
  • Easy placement of large objects
Models
Specifications
Accessories
Downloads

MODELS 10x/20x/40x PLPHFi objectives 100 W Halogen illumination 5 W LED illumination 100 W HBO fluorescence illumination  
EDI‑3053‑PLPHFi
EDI‑3053‑PLPHFi‑HAL  

EYEPIECES
Pair of DIN HWF plan 10x/22 mm eyepieces with diopter adjustment

HEAD
Trinocular 45° inclined tubes. Interpupillary distance of 47 to 78 mm. Trinocular head with 100-0, 80/20 and 0/100 positions

NOSEPIECE
Revolving sextuple nosepiece on ball-bearings. The nosepiece can be equipped with DIC prisms for the 10x/20x and 40x objectives

OBJECTIVES
High numerical infinity semi-apochromatic long working distance plan phase Fluarex PLPHFi 10x/0.30, 20x/0.45, S40x/0.60 IOS objectives enable the sub-cellular structures. An optional 60x/0.80 IOS objective is also available. The 20x, S40x and optional S60 objectives are equipped with collar adjustment ring for glass thicknesses from 0 to 1.2 mm

Fluarex™ objectives are made of low-absorption glass. Fluarex™ is a trademark of Euromex microscopen bv. All optics are anti-fungus treated and anti-reflection coated for maximum light throughput

STAGE
Stage 340 x 230 mm, equipped with a coaxial mechanical 130 x 85 mm X-Y stage. Supplied with:

  • Metal insert for O 38 mm cell culture dish
  • Metal insert for O 54 mm cell culture dish or 76 x 26 mm slides 
  • Metal insert for Multiwell plates (127.76  x 85.48 mm) 
  • Metal insert for Teraski plates (83.3 x 58.0 mm)
  • Optional universal cell culture holder

CONDENSER
Rotating N.A. 0.55 condenser with iris diaphragm. Working distance 26 mm. Supplied with phase contrast annuli for 10x/20x, 40x and 60x objectives and the DIC prisms for the 10x/20x/40x and 60x objectives. Three filter holders allow the insertion of Ø 38 mm filters in the optical path. The arm with condenser can be tilted backwards for convenient access to the stage. This allows easy placement of large objects like flasks and Petri dishes

LENS CHANGER
A lens changer with a 1.5x lens allow intermediary magnifications of 1.5 times the standard objective magnifications. Switching from 20x to 30x or 40 to 60 and from 60x to 90x magnification can be done without the need to re-focus on the sample

Delphi-X_Inverso_Detail-3Delphi-X_Inverso_Detail-4

6-Position fluorescence filter set wheel and 1.5 lens changer

FLUORESCENCE ATTACHMENT
The Delphi-X Inverso for fluorescence microscopy is supplied with a 6-position fluorescence filter set wheel and comes with two fluorescence filter sets for blue and green excitation (EDI-9745 and EDI-9746). Other filter sets are optional

DI.9745_grafiek

EDI-9745 Blue excitation, EX 460-495 DM 505 EM 510 long pass

 

DI.9746_grafiek

EDI-9746 Green excitation, EX 510-550 DM 570 EM 575 long pass

DI.9747_grafiek

EDI-9747 Violet excitation, EX 400-410 DM 455 EM 460 Long pass

DI.9748_grafiek

EDI-9748 Ultra violet excitation, EX 330-385 DM 410 EM 420 Long pass


DI.9750.TRITC_grafiek

EDI-9750.TRITC Green excitation, EX 528-553 DM 565 EM 578-633 band pass

DI.9750.FITC_grafiek

EDI-9750.FITC Blue excitation, EX 460-495 DM 505 EM 510-550 band pass

DI.9750.DAPI_grafiek

EDI-9750.DAPI UV excitation, EX 360-390 DM 415 EM 435-485 band pass

DI.9750.R_grafiek

EDI-9750.R Red excitation, EX 620-650 DM 660 EM 670-750 band pass

FOCUSING ADJUSTMENT
Coaxial 10 mm coarse and fine adjustments, 1 μm precision and with friction adjustment

ILLUMINATION
Fluorescence illumination with a 100 W mercury vapor lamp with external 100-240 V power supply. Transmitted 5 W LED or 100 W halogen illumination and internal power supply. Fuse holder 3.15 A / 250 V

PACKAGE CONTENT
Supplied with metal insert for Ø 38 mm cell culture dish and a metal insert for Ø 54 mm cell culture dish with 76 x 26 mm slides, metal insert for Multiwell plates, metal insert  for Teraski plates, blue and green interference Ø 45 mm filters, power cord, dust cover, green filter and alignment telescope and user manual

PB.5155 EPB-5155
Microscope slides 76 x 26 mm, ground edges, 50 pieces per pack
PB.5165 EPB-5165
Cover glasses 18 x 18 mm, 0.13-0.17 mm, 100 pieces
PB.5245 EPB-5245
Lens cleaning paper, 100 sheets per pack
PB.5274 EPB-5274
Iso propyl alcohol 99%, 200 ml
AE.5214 EAE-5214
Fuses 500 mA 250 V, per 10 pcs. models with LED
AE.5216 EAE-5216
Fuses 5A 250 V, per 10 pcs. models with 100 W halogen
PB.5168 EPB-5168
Cover glasses 22 x 22 mm, 0.13-0.17 mm. 100 pieces
DI.6010 EDI-6010
Super wide field SWF 10x/25 mm focusable eyepiece for Delphi-X Inverso, 30 mm diameter tube
No image EDI-6210
Super wide field SWF 10x/22 mm focusable eyepiece for Delphi-X Inverso, 30 mm diameter tube
No image EDI-6012
Wide field WF 12.5x/17.5 mm eyepiece for Ø 30 mm tube for Delphi-X Inverso
No image EDI-6015
Wide field WF 15x/16 mm eyepiece for Ø 30 mm tube for Delphi-X Inverso
No image EDI-6020
Wide field WF 20x/12 mm eyepiece for Ø 30 mm tube for Delphi-X Inverso
No image EDI-6099-L
Eyeshade for eyepieces
No image EDI-8704
Infinity Plan Semi-Apochromatic Phase contrast Fluarex PLPHFi-Apo 4x/0.13 objective, working distance 16,8 mm. Cover glass correction 1.2 mm. Needs phase annuli for 4x objective EDI-8404
DI.8710 EDI-8710
Infinity Plan Semi-Apochromatic Phase contrast Fluarex PLPHFi-Apo 10x/0.30 objective. Working distance 10.2 mm. Cover glass correction 1.2 mm
DI.8720 EDI-8720
Infinity Plan Semi-Apochromatic Phase contrast Fluarex PLPHFi-Apo 20x/0.45 objective with collar ring for adjustment of working distance between 7.5 to 8.8 mm. Cover glass correction 1.2 mm
No image EDI-9745-6
Standard filter set for Blue excitation EX 460-495 nm (BP) DM 505 EM 510 nm (LP). Needs empty cube
No image EDI-9746-6
Standard filter set for green excitation EX 510-550 nm (BP) DM 570 EM 575 (LP) Needs empty cube
No image EDI-9747-6
Standard filter set for Violet excitation EX 400-410 nm (BP) DM 455 EM 460 (LP) Needs empty cube
No image EDI-9748-6
Standard filter set for UV excitation EX 330-385 nm (BP) DM 410 EM 420 (LP) Needs empty cube
No image EDI-9749
Empty cube for Delphi-X Inverso Fluorescence attachment
DI.9750.19000 EDI-9750-19000
Fluorescence filter set EX375 (BP28: 361-389 nm) DM415 (LP) EM435 (LP) optimized for UV/DAPI, Calcofluor White. Needs empty filter block
DI.9750.19006 EDI-9750-19006
Fluorescence filter set EX560 (BP40: 540-580 nm) DM600 (LP) EM610 (LP) optimized for FM 4-64, Turbo FP650. Needs empty filter block
DI.9750.19008 EDI-9750-19008
Fluorescence filter set EX450 (BP50: 425-475 nm) DM485 (LP) EM495 (LP) optimized for Auramine Longpass. Needs empty filter block
DI.9750.19010 EDI-9750-19010
Fluorescence filter set EX480 (BP30: 465-495 nm) DM505 (LP) EM600 (LP) optimized for FM 1-43 / 4-64 , Pulsar 650 and Chlorophyll Longpass. Needs empty filter block
DI.9750.19011 EDI-9750-19011
Fluorescence filter set EX405 (BP30: 390-420 nm) DM505 (LP) EM450 (LP) optimized for Coumarin and Pacific Blue Longpass. Needs empty filter block
DI.9750.19012 EDI-9750-19012
Fluorescence filter set EX375 (BP28: 361-389 nm) DM415 (LP) EM515 (LP) optimized for FluoroGold and CalcoFluor White Longpass. Needs empty filter block
DI.9750.39000 EDI-9750-39000
Fluorescence filter set EX375 (BP28: 361-389 nm) DM415 (LP) EM460 (BP50: 435-485 nm) optimized for DAPI, Hoechst, AlexaFluor 350. Needs empty filter block
DI.9750.39001 EDI-9750-39001
Fluorescence filter set EX435 (BP20: 425-445 nm) DM455 (LP) EM480 (BP30: 465-495 nm) optimized for AmCyan, Atto 425, Cerulean, CFP, ECFP fluorophore. Needs empty filter block EIS-9749
DI.9750.39002 EDI-9750-39002
Fluorescence filter set EX480 (BP30: 465-495 nm) DM505(LP) EM535 (BP40: 515-555 nm) optimized for Acridine Orange + DNA, Alexa Fluor 488™, Atto 488, Azami Green, BODIPY FL/pH7.2, Calcein, Calcium, Green™-1, Cy2™, DiO, DyLight 488, EGFP, Emerald, GFP, FAM, FITC, Fluo-4, GFP, LysoTracker Green/pH 5.2, MitoTracker Green, FM/MeOH, mWasabi, Oregon Green™ 488, SYBR® Green I, SYTO 9/DNA, ZsGreen fluorophore. Needs empty filter block
DI.9750.39003 EDI-9750-39003
Fluorescence filter set EX495 (BP20: 485-505 nm) DM515 (LP) EM540 (BP30: 525-555 nm) optimized for Alexa Fluor 514™, Citrine, EYFP/pH 7, F/lAsH-CCPFCC, mCitrine, Oregon Green™ 514, Rhodamine 123, Topaz. Needs empty filter block
DI.9750.39004 EDI-9750-39004
Fluorescence filter set EX540 (BP25: 528-553 nm) DM565 (LP) EM605 (BP55: 578-633 nm) optimized for Alexa Fluor 546™, Alexa Fluor 555™, Atto 550, CAL, Fluor® Red 590, Cy3™, DiI, DsRed, DyLight 549, MitoTracker Orange/MeOH, Resorufin, Rhod-2, TagRFP, TAMRA tdTomato, Tetramethylrhodamine, isothiocyanate, TRITC. Needs empty filter block
DI.9750.39005 EDI-9750-39005
Fluorescence filter set EX540 (BP25: 528-552 nm) DM565 (LP) EM 620 (BP60: 590-650 nm) optimized for TRITC-red-shifted, Ethidium homidimer-1/DNA, Propidium Iodide. Needs empty filter block
DI.9750.39007 EDI-9750-39007
Fluorescence filter set EX620 (BP50: 595-645 nm) DM655 (LP) EM 690 (BP50: 665-715 nm) optimized for Alexa Fluor 647™, Allophycocyanin (APC), Atto 647N, Cy5™, DiD, Draq5, DyLight 649, MitoTracker Deep Red 633/MeOH, Nile Blue, Quasar® 670, SYTO® 60, TO-PRO™-3. Needs empty filter block
DI.9750.39008 EDI-9750-39008
Fluorescence filter set EX420 (BP40: 400-440) DM455 (LP) EM 535 (BP40: 515-555) optimized for Lucifer Yellow/Tetracycline/AlexaFluor 430. Needs empty filter block
DI.9750.39009 EDI-9750-39009
Fluorescence filter set EX480 (BP30: 465-495) DM505 (LP) EM 620 (BP60: 590-650) for Acridine Orange + RNA, Di-8-ANEPPS, non-radiometric, DiA, Ethidium Bromide, FM™ 1-43, LysoTracker Yellow HCK-123, Pulsar™ 650. Needs empty filter block
DI.9750.39010 EDI-9750-39010
Fluorescence filter set EX560 (BP40: 540-580) DM600 (LP) EM635 (BP60: 615-665) optimized for Texas Red, mCherry, AlexaFuor 594. Needs empty filter block EIS-9749
DI.9750.49000 EDI-9750-49000
Fluorescence filter set EX350 (BP50: 325-375) DM400 (LP) EM460 (BP50: 435-485) optimized for Texas Red, mCherry, AlexaFuor 594. Needs empty filter block EIS-9749
DI.9810 EDI-9810
C-mount with 1x magnification for C-mount camera
DI.9840 EDI-9840
C-mount with high resolution 0.40x objective for 1/3 inch C-mount camera
No image EDI-9850
C-mount with high resolution 0.50x objective for 1/2 inch C-mount camera